Phosphotyrosine | US Biological P4078-38 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine

catalog :

P4078-38

quantity :

50 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6D670

product information

Catalog Number :

P4078-38

Product wo Prefix :

Phosphotyrosine

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

50 ug

Clone # USB :

6D670

Isotype :

IgG1,k

Desc1 :

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains. Applications: Suitable for use in ELISA, Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 0.05ug/ml Western Blot (AP/BCIP/NBT (MTT)): 1ug/ml Western Blot (HRPO/ECL): 0.1ug/ml Immunoprecipitation: 1-10ug per 10e6 vanadate treated A431 cells Immunocytochemistry: 1-10ug/ml Optimal dilutions to be determined by the researcher. Western Blot Positive Control Included: P4078-38A: Phosphotyrosine, Molecular Weight Marker Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Ca Hu Mo Rt

Immunogen :

Phosphotyrosine conjugated to KLH

Specificity :

Recognizes phosphotyrosine in the context of the surrounding amino acids, showing high preference for phosphorylated EGF receptor. Species crossreactivity: human, mouse, rat, canine.

Purity :

Purified by size exclusion chromatography.

Form :

Supplied as a liquid in PBS, 0.09% sodium azide, PEG and sucrose.

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 6D670 Host: mouse Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, 0.09% sodium azide, PEG and sucrose. Purity: Purified by size exclusion chromatography. Immunogen: Phosphotyrosine conjugated to KLH Specificity: Recognizes phosphotyrosine in the context of the surrounding amino acids, showing high preference for phosphorylated EGF receptor. Species crossreactivity: human, mouse, rat, canine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IC IP WB

Storage Temperature :

-20°C