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product summary
company name :
US Biological
product type :
antibody
product name :
Phosphotyrosine
catalog :
P4078-26
quantity :
100 ug
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
product information
Catalog Number :
P4078-26
Product wo Prefix :
Phosphotyrosine
Host :
rabbit
Product Type :
Pab
Category :
Antibodies
Size1 :
100 ug
Isotype :
IgG
Desc1 :
Protein phosphorylation is a complex biochemical process that is regulated by protein kinases that catalyze the transfer of the gamma-P of ATP to a recipient protein that acts as a substrate. This post-translational modification is primarily directed onto serine, threonine, and tyrosine amino acid residues within a protein. Changes in the phosphorylation status of phosphoproteins can alter their biological function, and aberrations in signal transduction cascades have been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.
Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:250
ELISA: 1:1,000
Immunohistochemistry: 1:50
Immunoprecipitation: 10ug/500ug sample
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen :
Phosphotyrosine conjugated to KLH.
Specificity :
Recognizes proteins and peptides that contain phosphorylated tyrosine residues. Does not recognize phosphoserine, phosphothreonine or non-phosphorylated tyrosine.
Purity :
Purified by immunoaffinity chromatography
Form :
Supplied as a liquid in PBS, 0.01% sodium azide.
Concentration :
As reported
Desc2 :
Product Type: Pab
Isotype: IgG
Host: rabbit
Concentration: As reported
Form: Supplied as a liquid in PBS, 0.01% sodium azide.
Purity: Purified by immunoaffinity chromatography.
Immunogen: Phosphotyrosine conjugated to KLH.
Specificity: Recognizes proteins and peptides that contain phosphorylated tyrosine residues. Does not recognize phosphoserine, phosphothreonine or non-phosphorylated tyrosine.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Frackelton, A.R. Jr., Ross, A.H., and Eisen, H.N. (1983) Mol. Cell Biol. 3: 1343-1352.
2. Ross, A.H., Baltimore, D., and Eisen, H.N.(1981) Nature 294: 654-656.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/30/P4078-26_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
service@usbio.net
https://www.usbio.net800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.
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