Phosphotyrosine (FITC) | US Biological P4078-09 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine (FITC)

catalog :

P4078-09

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

4G10

product information

Catalog Number :

P4078-09

Product wo Prefix :

Phosphotyrosine (FITC)

Host :

mouse

Product Type :

Mab

Conjugate :

FITC

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

4G10

Isotype :

IgG2b,k

Desc1 :

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 5ug/ml. Detects phosphotyrosine containing proteins in EGF-stimulated human A431 cells. Optimal dilutions to be determined by researcher. Hybridoma: FOXY-NY (a NS-1 derivative) crossed with spleen cells from immunized mice. Storage and Stability Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Phosphotyramine (KLH coupled)

Specificity :

Recognizes phosphotyrosine and protein-phosphotyrosine. Does not react with phosphoserine or phosphothreonine.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.4. Labeled with fluorescein isothiocyanate (FITC).

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b,k Clone No: 4G10 Host: mouse Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.4. Labeled with fluorescein isothiocyanate (FITC). Purity: Purified by Protein G affinity chromatography. Immunogen: Phosphotyramine (KLH coupled) Specificity: Recognizes phosphotyrosine and protein-phosphotyrosine. Does not react with phosphoserine or phosphothreonine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Cohen, B., et al., PNAS USA 87: 4458-4462 (1990). 2. Druker, B.J., et al., New Eng. J. Med. 321: 1383-1391 (1989).