Phosphotyrosine (Biotin) | US Biological P4078-08A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine (Biotin)

catalog :

P4078-08A

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

4G10

product information

Catalog Number :

P4078-08A

Product wo Prefix :

Phosphotyrosine (Biotin)

Host :

mouse

Product Type :

Mab

Conjugate :

Biotin

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

4G10

Isotype :

IgG2b,k

Desc1 :

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: EGF-stimulated A431 cell lysate is provided as a free positive antigen control for Western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for immunoblot analysis. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Phosphotyramine-KLH

Specificity :

Recognizes tyrosine-phosphorylated proteins from all species.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b,k Clone No: 4G10 Host: mouse Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin. Purity: Purified by Protein G affinity chromatography. Immunogen: Phosphotyramine-KLH Specificity: Recognizes tyrosine-phosphorylated proteins from all species. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Cohen, B., et al., Proc. Natl. Acad. Sci. USA. 87: 4458-4462, 1990. 2. Druker, B.J., et al., New Eng. J. Med. 321: 1383-1391, 1989. 3. Kanakura, Y., et al., J. Biol. Chem. 266: 490-495, 1991.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/17/P4078-08A_1.jpg