antibody

Phosphotyrosine

P4078-05F

100 ug

monoclonal

mouse

nonconjugated

10F757

Phosphotyrosine

mouse

Mab

Antibodies

100 ug

10F757

IgG1

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8). Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Mo Rt

Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3’- dimentrylaminopropyl) carbodiimide.

Recognizes Phosphotyrosine. Does not cross react with phosphoserine or phosphothreonine. Species Crossreactivity: Not species specific.

Purified by Protein G affinity chromatography

Supplied as a liquid in PBS, pH7.4, 50% glycerol and 0.09% sodium azide.

~1mg/ml

Product Type: Mab Isotype: IgG1 Clone No: 10F757 Host: mouse Source: rat Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH7.4, 50% glycerol and 0.09% sodium azide. Purity: Purified by Protein G affinity chromatography Immunogen: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3’- dimentrylaminopropyl) carbodiimide. Specificity: Recognizes Phosphotyrosine. Does not cross react with phosphoserine or phosphothreonine. Species Crossreactivity: Not species specific. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF IHC IP WB

-20°C

1. Garton A.J., Flint A.J., and Tonks N.K. (1996) Mol and Cell Bio 16(11):6408-6418. 2. Garton A.J., and Tonks N.K. (1999) J Bio Chem. 274(6):3811-3818. 3. Goto H. et al. (2005) Nature Cell Biology 8:180-187. 4. Blume-Jensen P. and Hunter T. (2001) Nature 411:355-365. 5. Downward J. (2001) Nature 411:759-762. 6. Pawson T. and Saxton T.M. (1999) Cell 97:675-678. 7. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3:1343-1352. 8. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294:654-656. 9. Tiganis T., Kemp B.E., and Tonks N.K. (1999) J. Bio Chem. 274(39):27768-27775.