antibody

Phosphotyrosine discontinued

P4078-03

100 ug

polyclonal

domestic rabbit

nonconjugated

Phosphotyrosine discontinued

rabbit

Pab

Antibodies

100 ug

IgG

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains. Applications: Suitable for use in Western Blot, Slot Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:30,000 using colorimetric detection. Detection with more sensitive systems, such as chemiluminescence, may require higher antibody dilution. At a dilution of 1:1000 the antibody was easily able to detect 10ng (0.2pmol of a phosphorylated peptide) using chromogenic (BCIP/NBT) developement. Slot Blot: 1:10,000 ELISA: 1:30,000-1;100,000 for detection of 1ug/well of phosphotyrosine-containing substrate. Optimal dilutions to be determined by researcher. Positive Control: EGF-stimulated A431 cells containing high levels of phosphorylated EGF receptor Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

A random amino acid polymer containing phosphotyrosine (KLH coupled)

Specific for phosphotyrosine. Does not crossreact with phosphoserine, phosphothreonine, serine, threonine or tyrosine as confirmed by ELISA and Western Blot using phosphorylated and unphosphosphorylated protein. Species Crossreactivity: All species.

Purified by immunoaffinity chromatography

Supplied as a liquid in 0.05M Tris, 0.15M sodium chloride, pH 7.2, 0.02% sodium azide.

~5mg/ml

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~5mg/ml Form: Supplied as a liquid in 0.05M Tris, 0.15M sodium chloride, pH 7.2, 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: A random amino acid polymer containing phosphotyrosine (KLH coupled) Specificity: Specific for phosphotyrosine. Does not crossreact with phosphoserine, phosphothreonine, serine, threonine or tyrosine as confirmed by ELISA and Western Blot using phosphorylated and unphosphosphorylated protein. Species Crossreactivity: All species. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

4°C (-20°C Glycerol)

1. Nguyen, G., et al., J. Clin. Invest. 109(11): 1417-1427 (2002).