Phosphotyrosine | US Biological P4078-01C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine

catalog :

P4078-01C

quantity :

500 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10F280

product information

Catalog Number :

P4078-01C

Product wo Prefix :

Phosphotyrosine

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

500 ug

Clone # USB :

10F280

Isotype :

IgG2b

Desc1 :

The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of P4078-01C to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine. The affinity of P4078-01C for phosphotyrosine is 10e-6 to 10e-7 M. Applications: Suitable for use in ELISA, Immunofluorescence, and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 100ng/ml Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Phosphotyrosine

Specificity :

Recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in 100mM borate buffered saline, pH 8.0, 0.09% sodium azide.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 10F280 Host: mouse Concentration: ~0.5mg/ml Form: Supplied as a liquid in 100mM borate buffered saline, pH 8.0, 0.09% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Phosphotyrosine. Specificity: Recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF WB

Storage Temperature :

-20°C

Reference :

1. Ruff-Jamison, S. et al. (1991) Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features. J. Biol. Chem. 266: 6607-6613. 2. Takagi, S. et al. (1991) Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells. J. Exp. Med. 174: 381-388. 3. Glenney, J.R. Jr. et al. (1988) Monoclonal antibodies to phosphotyrosine. J. Immunol. Methods. 109: 277-285.