Phosphotyrosine | US Biological P4078-01A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine

catalog :

P4078-01A

quantity :

200 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5J256

product information

Catalog Number :

P4078-01A

Product wo Prefix :

Phosphotyrosine

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

200 ug

Clone # USB :

5J256

Isotype :

IgG2b

Desc1 :

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 1:2000 Western Blot: 1:2000 Immunohistochemistry: 1:500 Immunocytochemistry: 1:500 Immunoprecipitation: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Human phosphotyrosine coupled to carrier protein.

Specificity :

Recognizes human phosphorylated tyrosine residues. Does not crossreact with phosphorylated threonine or phosphorylated serine residues. Binding to phosphorylated tyrosine residues is inhibited by divalent cations at concentrations >1mM and salt concentrations >0.2M.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in PBS, pH 7.5, 3mM sodium azide, 50% glycerol.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 5J256 Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.5, 3mM sodium azide, 50% glycerol. Purity: Purified by immunoaffinity chromatography. Immunogen: Human phosphotyrosine coupled to carrier protein. Specificity: Recognizes human phosphorylated tyrosine residues. Does not crossreact with phosphorylated threonine or phosphorylated serine residues. Binding to phosphorylated tyrosine residues is inhibited by divalent cations at concentrations >1mM and salt concentrations >0.2M. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IC IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Druker, B.J., H.J. Mamon, and T.M. Roberts (1989) New Eng. J. Med. 321:1382-1391. 2. Frackelton, R.A., Jr., A.H. Ross, and H.N. Eisen (1983) Mol. Cell. Biol. 3:1343-1352. 3. Glenney, J.R., Jr., L. Zokas, and M.P. Kamps (1988) J. Immunol. Meth. 109:277-285. 4. Hanissian, S.H. and N. Sahyoun (1992) J. Neurosci. Res. 32(4):576-582. 5. Takagi, S., M. Daibata, T.J. Last, R.E. Humphreys, D.C. Parker, and T. Sairenji (1991) J. Exp. Med. 174(2):381-388. 6. Tremblay, L. and R. Beliveau (1994) Am. J. Physiol. 267(3 Pt 2):F415-F422. 7. Wang, J.Y.J. (1988) Anal. Biochem. 172:1-7.