antibody

Phosphothreonine

P4077

50 ug

polyclonal

domestic rabbit

nonconjugated

Phosphothreonine

rabbit

Pab

Antibodies

50 ug

IgG

Protein phosphorylation is a complex biochemical process that ultimately influences the mechanism by which a cell responds to a particular stimulus. A class of enzymes referred to as protein kinases regulates this process; these enzymes catalyze the transfer of the gama-P of ATP to a recipient protein that acts as a substrate. This post-translational modification is primarily directed onto serine and threonine amino acid residues within a protein. It has been noted that changes in the phosphorylation status of these phosphoproteins can alter the biological function of a protein. Additionally, aberrations in signal transduction cascade have been linked to a number of diseases including cancer, diabetes, heart disease, inflammation and neurological disorders. Applications: Suitable for use in Western Blot, ELISA, and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 2ug/ml; Mouse brain lysate. Incubate at RT for 4 hrs. ELISA: 0.5ug/ml (kinase assay). Binding of Ly-pT-Gly-HRP (0.2ug/100ul/well) to an anti-phosphothreonine coated microplate (0.5ug/100ul/well) is inhibited by 95% with the addition of 2-10ug/ml anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1mg/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Mo

Phosphothreonine conjugated to KLH and phosvitin.

Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins. Species crossreactivity: species independent

Purified by immunoaffinity chromatography

Supplied as a liquid in PBS, pH 7, 0.01% sodium azide, 50% glycerol.

~0.25mg/ml

Product Type: Pab Isotype: IgG Host: rabbit Source: mouse Concentration: ~0.25mg/ml Form: Supplied as a liquid in PBS, pH 7, 0.01% sodium azide, 50% glycerol. Purity: Purified by immunoaffinity chromatography. Immunogen: Phosphothreonine conjugated to KLH and phosvitin. Specificity: Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins. Species crossreactivity: species independent Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IP WB

-20°C

1. Frackelton, A.R., et al., Mol. Cell. Biol. 3: 1343 (1983). 2. Edelman, A.M., et al., Annu. Rev. Biochem. 56: 567 (1987). 3. Hunter, T., Cell 50: 823 (1987). 4. Glenny, J.R., et al., J. Immunological Meth. 109: 277 (1988). 5. Sengupta, A., et al., Proc. Natl. Acad. Sci. USA 85: 8062 (1988). 6. Heffetz, D., et al., Methods in Enzymology 210: 44 (1991). 7. Hunter, T., Methods in Enzymology 200: 3 (1991). 8. Rintamäki, E., et al., J. Biol. Chem. 272(48): 30,476–30,482 (1997). 9. Jin, D.Y., et al., Cell. 93: 81–91 (1998). 10. Reilein, A.R., et al., J. Biol. 142(3): 803–813 (1998). 11. Fleming, I.N., et al., J. Biol. Chem. 274(18): 12,753–12,758 (1999).

https://usbio-images.r.worldssl.net/prodimages/23/P4077_1.jpg