Phosphothreonine | US Biological P4077-16 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphothreonine

catalog :

P4077-16

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6D667 (4D11)

product information

Catalog Number :

P4077-16

Product wo Prefix :

Phosphothreonine

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

6D667 (4D11)

Isotype :

IgM

Desc1 :

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. Do not use milk or casein for blocking. Immunoprecipitation: 1-10ug per 10e6 pervanadate-treated A431 cells. ELISA: 0.05ug/ml Optmal dilutions to be determined by the researcher. Positive Control Included: P4076-22A: Phosphoserine/Phosphothreonine, Positive Control Add 100ul 2x SDS-PAGE sample buffer and incubate at 90°C for 5minutes. Western Blot: 20ul/lane HRPO/ECL Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Ca Hu Mo Rt

Immunogen :

Phosphothreonine containing peptides conjugated to KLH.

Specificity :

Recognizes a broad range of threonine-phosphorylated proteins. Species Crossreactivity: human, mouse, rat and canine.

Purity :

Purified by subsequent thiophillic adsorption and size exclusion chromatography.

Form :

Supplied as a liquid in PBS, 0.09% sodium azde, PEG and sucrose.

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Mab Isotype: IgM Clone No: 6D667 (4D11) Host: mouse Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, 0.09% sodium azde, PEG and sucrose. Purity: Purified by subsequent thiophillic adsorption and size exclusion chromatography. Immunogen: Phosphothreonine containing peptides conjugated to KLH. Specificity: Recognizes a broad range of threonine-phosphorylated proteins. Species Crossreactivity: human, mouse, rat and canine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/18/P4077-16_1.jpg