Phosphothreonine | US Biological P4077-11 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphothreonine

catalog :

P4077-11

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5H19

product information

Catalog Number :

P4077-11

Product wo Prefix :

Phosphothreonine

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

5H19

Isotype :

IgG1,k

Desc1 :

Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 to 1:5000, assayed against phosphothreonine conjugated BSA using IgG, H&L (HRP) Pab Gt xMo for detection. Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Phosphothreonine conjugated to KLH

Specificity :

Reacts specifically with phosphothreonine. Shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphotyrosine. Reacts with free phospho-amino acid, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell Reactivity is specific for phosphothreonine and minimal crossreactivity is observed against phosphoserine or phosphotyrosine lysates.

Purity :

Ascites

Form :

Supplied as a liquid, 0.01% sodium azide before the addition of glycerol to 40%. No buffer or stabilizers added. No stabilizing proteins added.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 5H19 Host: mouse Concentration: Not determined Form: Supplied as a liquid, 0.01% sodium azide before the addition of glycerol to 40%. No buffer or stabilizers added. No stabilizing proteins added. Purity: Ascites Immunogen: Phosphothreonine conjugated to KLH Specificity: Reacts specifically with phosphothreonine. Shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphotyrosine. Reacts with free phospho-amino acid, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell Reactivity is specific for phosphothreonine and minimal crossreactivity is observed against phosphoserine or phosphotyrosine lysates. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

4°C (-20°C Glycerol)

Reference :

1. Frackelton, AR, et al. (1983) Mol. Cell. Biol. 3:1343. 2. Edleman, AM, et al. (1987) Annu. Rev. Biochem. 56:567. 3. Hunter, T. (1987) Cell 50:823. 4. Sengupta, A, et al, (1988)