Phosphoserine | US Biological P4076-22 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphoserine

catalog :

P4076-22

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6D664 (7F12)

product information

Catalog Number :

P4076-22

Product wo Prefix :

Phosphoserine

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

6D664 (7F12)

Isotype :

IgG1

Desc1 :

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.05ug/ml Immunoprecipitation: 1-10ug/10e6 vanadate treated A431 cells Western Blot: 1ug/ml for HRPO/ECL detection. Recommended blocking buffer BPPT: 3%BSA, 1% PEG 4000, 1% PVP, 0.1% Tween 20, 2X PBS. Note: Do not use Milk/Casein for Blocking and dilution! Replace by BSA Optimal dilutions to be determined by the researcher. Positive Control Included: P4076-22A: Phosphoserine Positive Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Ca Hu Mo Rt

Immunogen :

Phosphoserine containing peptides.

Specificity :

Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts. The antibody has a preference for positively charged amino acids directly next to the phosphoserine. Please note that phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Species Crossreactivity: human, mouse, rat and canine.

Purity :

Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, PEG, sucrose.

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 6D664 (7F12) Host: mouse Source: human Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, PEG, sucrose. Purity: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography. Immunogen: Phosphoserine containing peptides. Specificity: Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts. The antibody has a preference for positively charged amino acids directly next to the phosphoserine. Please note that phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Species Crossreactivity: human, mouse, rat and canine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C