Phosphoserine | US Biological P4076-10G product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphoserine

catalog :

P4076-10G

quantity :

400 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

P4076-10G

Product wo Prefix :

Phosphoserine

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

400 ul

Isotype :

IgG

Desc1 :

Protein phosphorylation is an important post-translational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders. Applications: Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2-4ug/ml. Detects phosphorylation signal using human MMRU cells treated with 0.1uM okadaic acid. ELISA: 0.5-4ug/ml Immunoprecipitation: 10ug/mg Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Phosphoserine conjugated to KLH, and phosvitin mixture.

Specificity :

Recognizes proteins phosphorylated on serine residues. Does not crossreact with phosphotyrosine.

Purity :

Purified by affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.

Concentration :

~0.25mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~0.25mg/ml Form: Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol. Purity: Purified by affinity chromatography. Immunogen: Phosphoserine conjugated to KLH, and phosvitin mixture. Specificity: Recognizes proteins phosphorylated on serine residues. Does not crossreact with phosphotyrosine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Goto, H. et al. (2005). Nature Cell Biology 8: 180-187. 2. Blume-Jensen, P. and Hunter, T. (2001). Nature 411: 355-365. 3. Downward, J. (2001). Nature 411: 759-762. 4. Pawson, T. and Saxton, T.M. (1999). Cell 97: 675-678. 5. Ostrovsky, PC. (1995). Genes Dev. 9(16): 2034-2041.