Phosphoserine | US Biological P4076-01 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphoserine

catalog :

P4076-01

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3C171

product information

Catalog Number :

P4076-01

Product wo Prefix :

Phosphoserine

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

3C171

Isotype :

IgG1

Desc1 :

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated proteins, peptides, and antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family. Applications: Suitable for use in Western Blot, Dot Blot and Indirect ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot (Indirect): 1:500-1:1000 Indirect ELISA: 1:8000-1:16,000 Note: Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Optimal dilutions to be determined by researcher. Positive Control: Rat brain cortex and extract at ~50kD. Hybridoma: Mouse myeloma cells with splenocytes from immunized mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Phosphoserine conjugated to KLH.

Specificity :

Recognizes phosphorylated serine both as free amino acid or when coupled to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody because of steric hindrance of the recognition site. Species Crossreactivity: rat

Purity :

Ascites

Form :

Supplied as a liquid, 15mM sodium azide.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3C171 Host: mouse Concentration: Not Determined Form: Supplied as a liquid, 15mM sodium azide. Purity: Ascites Immunogen: Phosphoserine conjugated to KLH. Specificity: Recognizes phosphorylated serine both as free amino acid or when coupled to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody because of steric hindrance of the recognition site. Species Crossreactivity: rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

DB E WB

Storage Temperature :

-20°C

Reference :

1. Abu-Lawi, K.I. & Sultzer, B.M., Infect. Immunol. 63: 498-502 (1995). 2. Hunter, T. and Cooper, J.A., Annu. Rev. Biochem. 54: 897 (1985). 3. Heffetz, D., et al., Methods Enzymol. 201: 44 (1991). 4. Alexander, D. and Cantrell, D., Immunol. Today 10: 200 (1989). 5. Levine, L., et al., J. Immunol. Methods 124: 239 (1989).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/16/P4076-01_1.jpg