Phosphotyrosine (HRP) | US Biological P4075-39 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine (HRP)

catalog :

P4075-39

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

3G248

product information

Catalog Number :

P4075-39

Product wo Prefix :

Phosphotyrosine (HRP)

Host :

mouse

Product Type :

Mab

Conjugate :

HRP

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

3G248

Isotype :

IgG2b

Desc1 :

Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation, and differentiation(1, 2). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Optimal dilutions to be determined by the researcher. Positive controls: Mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cell lysates. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Phosphotyrosine coupled to KLH.

Specificity :

Reacts specifically with tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine. This antibody does not recognize phosphoserine or phosphothreonine. Species Crossreactivity: (with phosphorylated proteins) Independent of the protein’s species of orgin.

Purity :

Purified from mouse ascites

Form :

Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.1% proclin, 50% glycerol. Labeled with HRP.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 3G248 Host: mouse Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.1% proclin, 50% glycerol. Labeled with HRP. Purity: Purified from mouse ascites Immunogen: Phosphotyrosine coupled to KLH. Specificity: Reacts specifically with tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine. This antibody does not recognize phosphoserine or phosphothreonine. Species Crossreactivity: (with phosphorylated proteins) Independent of the protein’s species of orgin. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

4°C (-20°C Glycerol)

Reference :

Gerneral 1. Hunter, T.; Cell 50:823 (1987). 2. Glenney, J. R., Jr., et al; J Immunological Methods 109:277 (1988). Application 3. Greeberg. S., et al; J. Biol. Chem. 269(5):3897–3902 (1994). 4. Dash, D., et al; J. Biol. Chem. 270(29):17321–17326 (1995). 5. Raffioni, S. & Bradshow, R.A.; J. Biol. Chem. 270(13) 7568–7572 (1995). 6. Bourguignon, L.Y., et al; J. Biol. Chem. 272(44) 27913–27875 (1997). (cont’d) (03–7720 cont’d) 7. Jackson, D. ett al; J. Biol. Chem. 272(40): 24868–24875 (1997). Biacore 8. Zrihan-Licht, S. et al; J. Biol. Chem. 272(3): 1856–1863 (1997). 9. Raja, S. et al; J. Biol. Chem. 272(16): 10941–10947 (1997). 10. Jackson, D. et al; J. Biol. Chem. 272(11): 6986–6993 (1997). 11. Lawler, S., et al; J. Biol. Chem. 272(23):14850–14859 (1997). 12. Chen, J.K., et al; J. Biol. Chem. 273(44):29254–29261 (1998).