Phosphotyrosine (AP) | US Biological P4075-38 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine (AP)

catalog :

P4075-38

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

3G247

product information

Catalog Number :

P4075-38

Product wo Prefix :

Phosphotyrosine (AP)

Host :

mouse

Product Type :

Mab

Conjugate :

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

3G247

Isotype :

IgG2b

Desc1 :

Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation and differentiation. Antibodies to phosphotyrosine have proven to be useful in the characterization of these kinase substrates that may be involved in oncogenesis and regulation of cell growth. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 2-10ug/IP reaction Note: Binding is inhibited by high salt concentrations and by divalent cations. Use a buffer with less than 150mM sodium chloride and divalent cations not much greater than 1mM. Optimal dilutions to be determined by the researcher. Positive Control: Mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cell lysates. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo

Immunogen :

Phosphotyrosine coupled to KLH.

Specificity :

Recognizes native and denatured tyrosine phosphorylated proteins Binding of this antibody is inhibited by phosphotyrosine. Will not react with phosphoserine or phosphothreonine residues on proteins. Species Crossreactivity: human and mouse.

Purity :

Purified

Form :

Supplied as a liquid in TBS, 1% BSA, 1mM magnesium chloride, 0.09% sodium azide, 50% glycerol. Labeled with alkaline phosphatase (AP).

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 3G247 Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in TBS, 1% BSA, 1mM magnesium chloride, 0.09% sodium azide, 50% glycerol. Labeled with alkaline phosphatase (AP). Purity: Purified Immunogen: Phosphotyrosine coupled to KLH. Specificity: Recognizes native and denatured tyrosine phosphorylated proteins Binding of this antibody is inhibited by phosphotyrosine. Will not react with phosphoserine or phosphothreonine residues on proteins. Species Crossreactivity: human and mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

4°C Do Not Freeze

Reference :

1. Glenney, J.R., Jr., et al., J. Immunol. Methods 109: 277 (1988). 2. Ruff-Jamison, S., et al., J. Biol. Chem. 266: 6607–6613 (1991). 3. Zrihan-Licht, S., et al., J. Biol. Chem. 272(3): 1856–1863 (1997). 4. Raja, S., et al., J. Biol. Chem. 272(16): 10,941–10,947 (1997). 5. Jackson, D., et al., J. Biol. Chem. 272(11): 6986–6993 (1997). 6. Jackson, D., et al., J. Biol. Chem. 272(40): 24,868–24,875 (1997). 7. Greebera. S., et al., J. Biol. Chem. 269(5): 3897–3902 (1994). 8. Dash, D., et al., J. Biol. Chem. 270(29): 17,321–17,326 (1995). 9. Bourguignon, L.Y., et al., J. Biol. Chem. 272(44): 27,913–27,875 (1997). 10. Raffioni, S. & Bradshow, R.A., J. Biol. Chem. 270(13): 7568–7572 (1995). 11. Lawler, S., et al., J. Biol. Chem. 272(23): 14,850–14,859 (1997). 12. Chen, J.K., et al., J. Biol. Chem. 273(44): 29,254–29,261 (1998).