antibody

Phosphotyrosine, mixed clones

P4075-35

100 ug

monoclonal

mouse

nonconjugated

PY-7E1, PY20

Phosphotyrosine, mixed clones

mouse

Mab

Antibodies

100 ug

PY-7E1, PY20

IgG1,k

Protein tyrosine phosphorylation plays a central role in the transmission of signals from the cell surface to the nucleus.(3,7) Reversible phosphorylation of cellular proteins on specific tyrosine residues is involved in a variety of fundamental cellular processes, including survival, proliferation, differentiation, and transformation.(3,6,7) The use of antibodies which specifically recognize phosphotyrosine is widely accepted as one of the most powerful tools for detection, characterization, and purification of proteins that are either constitutively or inducibly phosphorylated on tyrosine (where even a single phosphorylated tyrosine residue is sufficient for detection).(1,2,4,5) Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Tyrosine-phosphorylated proteins

PY-Plus reacts specifically with tyrosine-phosphorylated proteins. It shows no crossreactivity to phosphoserine or phosphothreonine. On Western blots, specific inhibition of each individual antibody and the cocktail reactivity can be achieved by pre-incubation of the antibody with 20 mM phosphotyrosine, whereas pre-incubation with 20 mM phosphoserine or 20 mM phosphothreonine is ineffective. Species crossreactivity: It is well established that no one monoclonal antibody can strongly react with all tyrosine-phosphorylated proteins. PY-Plus contains 2 different monoclonal antibodies that are designed to strongly react with the broadest range of tyrosinephosphorylated proteins possible. It will react with tyrosine-phosphorylated proteins in both the native and denatured states. Positive reactivity is independent of the species source and has been observed in the following samples: mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cells.

Purified by immunoaffinity chromatography

Supplied as a liquid in PBS, pH 7.4, 0.1% Proclin, 40% glycerol.

As reported

Product Type: Mab Isotype: IgG1,k Clone No: PY-7E1, PY20 Host: mouse Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.4, 0.1% Proclin, 40% glycerol. Purity: Purified by immunoaffinity chromatography. Immunogen: Tyrosine-phosphorylated proteins Specificity: PY-Plus reacts specifically with tyrosine-phosphorylated proteins. It shows no crossreactivity to phosphoserine or phosphothreonine. On Western blots, specific inhibition of each individual antibody and the cocktail reactivity can be achieved by pre-incubation of the antibody with 20 mM phosphotyrosine, whereas pre-incubation with 20 mM phosphoserine or 20 mM phosphothreonine is ineffective. Species crossreactivity: It is well established that no one monoclonal antibody can strongly react with all tyrosine-phosphorylated proteins. PY-Plus contains 2 different monoclonal antibodies that are designed to strongly react with the broadest range of tyrosinephosphorylated proteins possible. It will react with tyrosine-phosphorylated proteins in both the native and denatured states. Positive reactivity is independent of the species source and has been observed in the following samples: mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cells. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

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