Phosphotyrosine | US Biological P4075-33 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine

catalog :

P4075-33

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

P4075-33

Product wo Prefix :

Phosphotyrosine

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the posttranslational state of a broad range of phosphotyrosine containing proteins. Immunoprecipitation of proteins of interest followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation or for antigen applications in immunological protocols. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs.

Specificity :

Detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Host: rabbit Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs. Specificity: Detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1981 Ross, A.H. et al. Nature 294:654. 1988 Wang, J.Y.J. Anal. Biochem 172:1.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/48/P4075-33_1.jpg