Phosphotyrosine | US Biological P4075-32 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine

catalog :

P4075-32

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

P4075-32

Product wo Prefix :

Phosphotyrosine

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation and differentiation. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Can also be used for conjugation to label including enzymes, fluorochromes, biotin and agarose. Other applications not tested. Recommended Dilutions: ELISA: 1:5000-10,000 Western Blot: 1:1000-1:2000 Immunoprecipitation: 2-5ug/IP reaction Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Affinity purified native tyrosine phosphorylated proteins

Specificity :

Recognizes human Phosphotyrosine. Specific to phosphotyrosine containing proteins. Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins. Species Crossreactivity: tyrosine-phosphorylated proteins from any species

Purity :

Purified by affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Concentration :

~0.25mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~0.25mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Purity: Purified by affinity chromatography. Immunogen: Affinity purified native tyrosine phosphorylated proteins Specificity: Recognizes human Phosphotyrosine. Specific to phosphotyrosine containing proteins. Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins. Species Crossreactivity: tyrosine-phosphorylated proteins from any species Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. Hunter, T., Cell 50: 823 (1987). 2. Sorkin, A. & Carpenter, G., Science 261: 612-615 (1993). 3. Soler, C., et al., J. Biol. Chem. 269: 12,320-12,324 (1994). 4. Kiener, P.A., et al., J. Immunology 155: 4917-4925 (1995). 5. Khurana, S., et al., J. Biol. Chem. 271: 9919-9927 (1996). 6. Khurana, S., et al., J. Biol. Chem. 272: 30,115-30,121 (1997). 7. Palumbo, G.A., et al., Cancer Research 57: 2434-2439 (1997). 8. Khurana, S., et al., J. Biol. Chem. 271: 9919-9927 (1996). 9. Poppleton, H., et al., J. Biol. Chem. 271: 6947-6951 (1996). 10. Chen, Z., et al., J. Biol. Chem. 270: 27,525-27,530 (1995). 11. Chen, J.K., et al., J. Biol. Chem. 274: 4764-4769 (1999). 12. Chen, J.K., et al., J.Biol. Chem. 273: 29,254-29,261(1998). 13. Yipt, J.W., et al., J. Biol. Chem. 272: 18,473-18,480 (1997). 14. Maruyama, T., et al., Endocrinology 140: 5982-5990 (1999).