Phosphotyrosine (HRP) | US Biological P4075-29C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine (HRP)

catalog :

P4075-29C

quantity :

400 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

HRP

product information

Catalog Number :

P4075-29C

Product wo Prefix :

Phosphotyrosine (HRP)

Host :

rabbit

Product Type :

Pab

Conjugate :

HRP

Category :

Antibodies

Size1 :

400 ul

Isotype :

IgG

Desc1 :

Protein phosphorylation is an important post-translational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Phosphotyrosine conjugated to KLH

Specificity :

Recognizes proteins and peptides that contain phosphorylated tyrosine residues. Does not crossreact with phosphoserine or threonine.

Purity :

Purified

Form :

Supplied as a liquid in TBS, 0.01% preservative. Labeled with horseradish peroxidasae (HRP).

Concentration :

~0.25mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~0.25mg/ml Form: Supplied as a liquid in TBS, 0.01% preservative. Labeled with horseradish peroxidasae (HRP). Purity: Purified Immunogen: Phosphotyrosine conjugated to KLH Specificity: Recognizes proteins and peptides that contain phosphorylated tyrosine residues. Does not crossreact with phosphoserine or threonine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C (-20°C Glycerol)

Reference :

1. Goto, H. et al. (2005). Nature Cell Biology 8: 180-187. 2. Blume-Jensen, P. and Hunter, T. (2001). Nature 411: 355-365. 3. Downward, J. (2001). Nature 411: 759-762. 4. Pawson, T. and Saxton, T.M. (1999). Cell 97: 675-678. 5. Frackelton, A.R. Jr., Ross, A.H., and Eisen, H.N. (1983). Mol Cell Biol. 3: 1343-1352. 6. Ross, A.H., Baltimore, D., and Eisen, H.N. (1981). Nature 294: 654-656. 7. Ostrovsky, PC. (1995). Genes Dev. 9(16): 2034-2041.