antibody

Phosphotyrosine

P4075-28

1 mg

monoclonal

mouse

nonconjugated

3G239

Phosphotyrosine

mouse

Mab

Antibodies

1 mg

3G239

IgG2b

Specific reversible protein phosphorylation plays a central role in regulating many cellular events by controlling the activity of participating proteins. Ways that phosphorylation/dephosphorylation can modulate a protein’s activity include altering its conformation, localization, stability, and ability to associate or form complexes with other proteins(3-5). As much as one third of cellular proteins may be phosphorylated at any given moment. Reversible protein tyrosine phosphorylations are central to regulating events in signal transduction pathways initiated by most growth factors and cytokines. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunoprecipitation: 2-5ug Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

KLH coupled with equal amounts of phosphotyrosine, glycine, and alanine.

Recognizes specifically with tyrosine phosphorylated proteins in the native or denatured states. It does not react with phosphoserine or phosphothreonine.(1) Binding of PY20 to phosphotyrosine containing proteins or peptides is inhibited by free phosphotyrosine. Species Crossreactivity: Recognition of phosphotyrosine in peptides or proteins is independent of species of origin.

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM sodium phosphate, pH 7.4, 0.05% sodium azide.

~0.25mg/ml

Product Type: Mab Isotype: IgG2b Clone No: 3G239 Host: mouse Concentration: ~0.25mg/ml Form: Supplied as a liquid in 10mM sodium phosphate, pH 7.4, 0.05% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: KLH coupled with equal amounts of phosphotyrosine, glycine, and alanine. Specificity: Recognizes specifically with tyrosine phosphorylated proteins in the native or denatured states. It does not react with phosphoserine or phosphothreonine.(1) Binding of PY20 to phosphotyrosine containing proteins or peptides is inhibited by free phosphotyrosine. Species Crossreactivity: Recognition of phosphotyrosine in peptides or proteins is independent of species of origin. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Glenney Jr., J.R., et al, J. Immunol. Methods 109:277 (1988). 2. Sargiacomo, M., et al, J. Cell Biol. (1993). 3. Hunter, T., Meth. Enzymol. 200:3–37 (1991). 4. Hunter, T., Cell 50:823–829 (1987). 5. Hunter, T., Cell 80:225–236 (1987). 6. Bourguignon, L.Y., et al., J. Biol. Chem. 272:27913–27918 (1997). 7. Jackson, D. et al, J. Biol. Chem. 272(11): 6986–6993 (1997). 8. Raffioni, S. and Bradshaw, R.A., J. Biol, Chem, 270:7568–7572 (1995). 9. Raja, S. et al, J. Biol. Chem. 272(16): 10941–10947 (1997). 10. Zrihan-Licht, S. et al. J. Biol. Chem. 272(3): 1856–1863 (1997). 11. Jackson, D. et al, J. Biol. Chem. 272(40): 24868–24875 (1997). 12. Jackson, D. et al; J. Biol. Chem. 272(11): 6986–6993 (1997). 13. Greebera. S., et al; J. Biol. Chem. 269(5):3897–3902 (1994). 14. Dash, D., et al; J. Biol. Chem. 270(29):17321–17326 (1995). 15. Lawler, S., et al; J. Biol. Chem. 272(23):14850–14859 (1997). 16. Chen, J.K., et al; J. Biol. Chem. 273(44):29254–29261 (1998).