Phosphotyrosine | US Biological P4075-27 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine

catalog :

P4075-27

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P6V6

product information

Catalog Number :

P4075-27

Product wo Prefix :

Phosphotyrosine

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

P6V6

Isotype :

IgG1

Desc1 :

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:10, can be performed using standard procedures. Blocking should be performed with 1-1.5% bovine serum albumin (for phosphotyrosine blotting never use non-fat dry milk or other). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Specificity :

Recognizes phosphotyrosine. It does not crossreact with phosphoserine or phosphothreonine.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: P6V6 Host: mouse Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Purity: Purified by Protein G affinity chromatography. Specificity: Recognizes phosphotyrosine. It does not crossreact with phosphoserine or phosphothreonine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C