Phospholipase D1, phosphorylated (Thr147) (PLD1) | US Biological P4074-50B product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Phospholipase D1, phosphorylated (Thr147) (PLD1)

catalog :

P4074-50B

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

P4074-50B

Product wo Prefix :

Phospholipase D1, phosphorylated (Thr147) (PLD1)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger, diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified. Both are regulated by protein kinases, small GTPases and Ca2+. PLD1 is phosphorylated at Ser2, Ser561 and Thr147 by PKC. Phosphorylation at Ser147 and Thr561 activates PLD1 activity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Band is observed at ~120kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Thr147 of human PLD1.

Specificity :

Detects endogenous levels of human PLD1 phosphorylated at Thr147. Does not crossreact with phosphorylated PLD2. Species Crossreactivity: mouse and rat

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr147 of human PLD1. Specificity: Detects endogenous levels of human PLD1 phosphorylated at Thr147. Does not crossreact with phosphorylated PLD2. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Exton, J.H., Biochim. Biophys. Acta 1439: 121–133 (1999). 2. Kim, Y., et al., Biochemistry 38: 10,3244–10,351 (1999). 3. Kim, Y., et al., J. Biol. Chem. 275: 13,621–13,627 (2000).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/27/P4074-50B_1.jpg