c-Phospholipase A2, phosphorylated (Ser505) (Cytosolic PLA2, cPLA2) | US Biological P4074-10 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

c-Phospholipase A2, phosphorylated (Ser505) (Cytosolic PLA2, cPLA2)

catalog :

P4074-10

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

P4074-10

Product wo Prefix :

c-Phospholipase A2, phosphorylated (Ser505) (Cytosolic PLA2, cPLA2)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and the release of arachidonic acid. cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity. Calcium binding to the Nterminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane. Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at serine 505 and serine 727 stimulates its catalytic activity. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser505 of human cPLA2.

Specificity :

Recognizes endogenous levels of human cPLA2 only when phosphorylated at Ser505, Mr ~95kD. It does not crossreact with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2). Species Crossreactivity: mouse, rat and monkey

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser505 of human cPLA2. Specificity: Recognizes endogenous levels of human cPLA2 only when phosphorylated at Ser505, Mr ~95kD. It does not crossreact with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2). Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Leslie, C.C. (1997) J. Biol. Chem. 272, 16709-16712. 2. Clark, J.D. et al. (1991) Cell 65, 1043-1051. 3. Lin, L.L. et al. (1993) Cell 72, 269-278. 4. Kramer, R.M. (1996) J. Biol. Chem. 271, 27723-27729. 5. Hefner, Y. et al. (2000) J. Biol. Chem. 275, 37542-37551.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/5/P4074-10_1.jpg