Phosphoinositide 3 Kinase, p85 (PI3K) | US Biological P4073-29A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphoinositide 3 Kinase, p85 (PI3K)

catalog :

P4073-29A

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

19H8

The same clone is also sold as:

Cell Signaling Technology: 4257

reactivity :

human

product information

Catalog Number :

P4073-29A

Product wo Prefix :

Phosphoinositide 3 Kinase, p85 (PI3K)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

19H8

Isotype :

IgG

Desc1 :

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol- 4-phosphate (PIP) and phosphatidylinositol-4,5- bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110 alpha, p110 beta, p110 delta and p110 gamma) have been isolated, and the regulatory subunits that associate with p110 alpha, p110 beta and p110 delta are p85 alpha and p85 beta (3). In contrast, p110 gamma associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110 gamma is activated by beta gamma subunits of heterotrimeric G proteins (4). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot 1:1000 Immunoprecipitation 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to the sequence of human PI3K p85.

Specificity :

Detects endogenous levels of total PI3K p85 protein. Species Crossreactivity: Human, mouse and rat.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 19H8 Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the sequence of human PI3K p85. Specificity: Detects endogenous levels of total PI3K p85 protein. Species Crossreactivity: Human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

(1) Cantley, L.C. (2002) Science 296, 1655–1657. (2) Simpson, L. and Parsons, R. (2001) Exp. Cell Res. 264, 29–41. (3) Neri, L.M. et al. (2002) BBA 1584, 73–80. (4) Stoyanov, B. et al. (1995) Science 269, 690–693.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/14/P4073-29A_1.jpg