antibody

PHD3 (Prolyl Hydroxylase Domain Containing Protein 3, HIF-Prolyl Hydroxylase 3, EGLN3)

P3375-05

100 ul

polyclonal

domestic rabbit

nonconjugated

PHD3 (Prolyl Hydroxylase Domain Containing Protein 3, HIF-Prolyl Hydroxylase 3, EGLN3)

rabbit

Pab

Antibodies

100 ul

IgG

HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-1, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(564) and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may also play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissues, promoting cell death through a caspase-dependent mechanism. Applications: Suitable for use in Western Blot., Immunohistochemistry, Immunofluorescence/Immunocytochemistry, Immunoprecipitation and Electron Microscopy. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 Immunofluorescence (IC): 1:500 Immunohistochemistry (Paraffin): 1:250-1:1000 Optimal dilution to be determined by the researcher. Positive Control: HeLa cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Rt

A synthetic peptide corresponding to a region between residues 50-100 of human PHD3 (NP_071356.1, GeneID 112399), Species Sequence Homology: mouse

Recognizes human PHD3 at ~28kD. Species Crossreactivity: rat

Purified by immunoaffinity chromatography

Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide

~1mg/ml

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide Purity: Purified by immunoaffinity chromatography Immunogen: A synthetic peptide corresponding to a region between residues 50-100 of human PHD3 (NP_071356.1, GeneID 112399), Species Sequence Homology: mouse Specificity: Recognizes human PHD3 at ~28kD. Species Crossreactivity: rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IC IF IHC IP WB

-20°C

1. Bruick, RK, McKnight, SL. A Conserved Family of Prolyl-4-Hydroxylases That Modify HIF. Science. 294:1337-1340(2001). 2. Ivan, M, et al. Biochemical Purification and Pharmacological Inhibition of a Mammalian Prolyl Hydroxylase Acting on Hypoxia-Inducible Factor. PNAS. 99(21):13459-13464(2002). 3. Maxwell, PH, Ratcliffe, PJ. Oxygen Sensors and Angiogenesis. Cell & Dev. Bio. 13:29-37(2002).

https://usbio-images.r.worldssl.net/prodimages/0/P3375-05_1.jpg

Detection of PHD3 by Western Blot application in lysate from Rat cells before or 9 hours after inducing hypoxia. P3375-05 was used at a dilution of 1:5000 and blots were developed using chemiluminescence.