PF4 (Platelet Factor 4) (Carboxyfluorescein) | US Biological P3360-01D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PF4 (Platelet Factor 4) (Carboxyfluorescein)

catalog :

P3360-01D

quantity :

100Tests

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H2

reactivity :

human

product information

Catalog Number :

P3360-01D

Product wo Prefix :

PF4 (Platelet Factor 4) (Carboxyfluorescein)

Host :

mouse

Product Type :

Mab

Conjugate :

Carboxyfluorescein

Category :

Antibodies

Size1 :

100Tests

Clone # USB :

7H2

Isotype :

IgG2b

Desc1 :

PF4 (Platelet Factor 4, CXCL4) is a member of the CXC chemokine family. Human PF4 is a 101 amino acid(aa) protein with a 32aa signal sequence and a 70aa mature protein. PF4 has homology with IL-8 and b-thromboglobulin. Human and mouse PF4 share a 64% identity. PF4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual PF4 subunits. Megakaryocytes synthesize PF4 and store it as tetramers in alpha- granules. The PF4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, PF4 lacks chemotactic activity for polymorphonuclear granulocytes. PF4 does not contain an ELR motif. However, many other functions have been observed for PF4. PF4 is involved in monocyte survival and differentiation into macrophages, and it has anti-angiogenic activity. PF4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors, contributing to its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as PF4 binding sites; affinity is controlled by the degree of sulfation of these chains (1-6). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul label ≤ 5x10e5 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to human PF4, Glu32-Ser101, expressed in E. coli.

Specificity :

Recognizes human CXCL4/PF4.

Purity :

Purified by Protein G affinity chromatography from cell culture supernatant.

Form :

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 7H2 Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein. Purity: Purified by Protein G affinity chromatography from cell culture supernatant. Immunogen: Recombinant protein corresponding to human PF4, Glu32-Ser101, expressed in E. coli. Specificity: Recognizes human CXCL4/PF4. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Poncz, M. et al. (1987) Blood 69:219. 2. Scheuerer, B. et al. (2000) Blood 95:1158. 3. Perollet, C. et al. (1998) Blood 91:3289. 4. Petersen, F. et al. (1998) J. Immunol. 161:4347. 5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376. 6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/1/P3360-01D_1.jpg