PDK-1 (Pyruvate Dehydrogenase Kinase-1, PDHK1) | US Biological P3123-10B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PDK-1 (Pyruvate Dehydrogenase Kinase-1, PDHK1)

catalog :

P3123-10B

quantity :

20 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

10E44(C47H1)

reactivity :

human

product information

Catalog Number :

P3123-10B

Product wo Prefix :

PDK-1 (Pyruvate Dehydrogenase Kinase-1, PDHK1)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ul

Clone # USB :

10E44(C47H1)

Isotype :

IgG

Desc1 :

Pyruvate generated from glycolysis is converted to acetyl-CoA by pyruvate dehydrogenase (PDH) under normoxia (1-3). This is a critical link between glycolysis and the TCA cycle (3). PDH activity is regulated by phosphorylation and dephosphorylation (3). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (3). Hypoxia can directly induce pyruvate dehydrogenase kinase 1 (PDHK1) expression, which results in inactivation of PDH and the TCA cycle and subsequent suppression of metabolism (4). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the sequence of human PDHK1. Species sequence homology: bovine (100%)

Specificity :

Recognizes endogenous levels of total human PDHK1. Species crossreactivity: mouse, rat, monkey.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 10E44(C47H1) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the sequence of human PDHK1. Species sequence homology: bovine (100%) Specificity: Recognizes endogenous levels of total human PDHK1. Species crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Harris, R.A. et al. (2002) Adv Enzyme Regul 42, 249-59, 2. Fries, M. et al. (2003) Biochemistry 42, 6996-7002, 3. Wigfield, S.M. et al. (2008) Br J Cancer 98, 1975-84, 4. Kim, J.W. et al. (2006) Cell Metab 3, 177-85.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/25/P3123-10B_1.jpg