PDK1 (PDPK1, PI–P3 Dependent Kinase, 3a Phosphoinositide-dependent Protein Kinase 1) | US Biological P3123-09 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PDK1 (PDPK1, PI–P3 Dependent Kinase, 3a Phosphoinositide-dependent Protein Kinase 1)

catalog :

P3123-09

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P3123-09

Product wo Prefix :

PDK1 (PDPK1, PI–P3 Dependent Kinase, 3a Phosphoinositide-dependent Protein Kinase 1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

PDK-1 (3-Phosphoinositide-dependent Protein Kinase-1) phosphorylates AGC kinases. PDK-1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK-1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase-3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:750 Western Blot: 1:750 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

A synthetic peptide, RKIQEVWRQRYQS, corresponding to amino acids 301-312 of PDK-1. Accession number AC005591.

Specificity :

Recognizes human PDK-1 (60kD).

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as liquid in PBS, 0.09% sodium azide. No stabilizing proteins added.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~1mg/ml Form: Supplied as liquid in PBS, 0.09% sodium azide. No stabilizing proteins added. Purity: Purified by immunoaffinity chromatography. Immunogen: A synthetic peptide, RKIQEVWRQRYQS, corresponding to amino acids 301-312 of PDK-1. Accession number AC005591. Specificity: Recognizes human PDK-1 (60kD). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

4°C (-20°C Glycerol)

Reference :

1.Biondi RM, et al., EMBO J. 15;21(16):4219-28.(2002).2.Fujita N, et al., . J Biol Chem. 22;277(12):10346-53.(2002) 3.Park J, J Biol Chem. 5;276(40):37459-71.(2001)4.Scheid MP, et al. Mol Cell Biol.22(17):6247-60.(2002) 5.Wick MJ, et al. J Biol Chem. 10;277(19):16632-8.(2002)