PDPK1, phosphorylated (Ser241) (3-phosphoinositide-dependent Protein Kinase 1, hPDK1, PDK1) discontinued | US Biological P3123-02V product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PDPK1, phosphorylated (Ser241) (3-phosphoinositide-dependent Protein Kinase 1, hPDK1, PDK1) discontinued

catalog :

P3123-02V

quantity :

50 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

P3123-02V

Product wo Prefix :

PDPK1, phosphorylated (Ser241) (3-phosphoinositide-dependent Protein Kinase 1, hPDK1, PDK1) discontinued

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

50 ug

Isotype :

IgG

Desc1 :

PDK1 or PDPK1 is a 63kD monomeric protein which is also known as Akt kinase. PDK1 is composed of a C-terminal PH (pleckstrin homology) domain and an N-terminal catalytic domain. In response to insulin or insulin-like growth factor, PDK1 phosphorylates PKB/Akt1 on threonine 308 and serine 473 in a phosphatidylinositol 3,4,5-triphosphate or phosphatidylinositol 3,4-biphosphate-dependent manner. Phosphatidylinositol 3,4,5-triphosphate or phosphatidylinositol 3,4-biphosphate binds to the PH domains of PKB and PDK1 resulting in the translocation of these proteins to the cell membrane and activation of PKB. PDK1 also phosphorylates the 70kD ribosomal protein S6 kinase at threonine 229, which is required for its activation. Thus, PDK1 acts upstream of PKB and has been shown to control signals for proliferation and apoptosis. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic phosphopeptide corresponding to aa236-248 of human PDPK1, pSer241 (KLH).

Specificity :

Recognizes human PDPK1when phosphorylated at Ser241.

Purity :

Purified

Form :

Supplied as a liquid in PBS, 0.09% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Host: rabbit Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 0.1% sodium azide. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to aa236-248 of human PDPK1, pSer241 (KLH). Specificity: RRecognizes human PDPK1when phosphorylated at Ser241. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/14/P3123-02V_1.jpg