PDK1 (3-phosphoinositide Dependent Protein Kinase-1, hPDK1, PDK1) | US Biological P3123-02U product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PDK1 (3-phosphoinositide Dependent Protein Kinase-1, hPDK1, PDK1)

catalog :

P3123-02U

quantity :

50 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P3123-02U

Product wo Prefix :

PDK1 (3-phosphoinositide Dependent Protein Kinase-1, hPDK1, PDK1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

50 ug

Isotype :

IgG

Desc1 :

PDK1 or PDPK1 is a 63kD monomeric protein which is also known as Akt kinase. PDK1 is composed of a C-terminal PH (pleckstrin homology) domain and an N-terminal catalytic domain. In response to insulin or insulin-like growth factor, PDK1 phosphorylates PKB/Akt1 on threonine 308 and serine 473 in a phosphatidylinositol 3,4,5-triphosphate or phosphatidylinositol 3,4-biphosphate-dependent manner. Phosphatidylinositol 3,4,5-triphosphate or phosphatidylinositol 3,4-biphosphate binds to the PH domains of PKB and PDK1 resulting in the translocation of these proteins to the cell membrane and activation of PKB. PDK1 also phosphorylates the 70kD ribosomal protein S6 kinase at threonine 229, which is required for its activation. Thus, PDK1 acts upstream of PKB and has been shown to control signals for proliferation and apoptosis. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence:1:100-1:200 Western Blot:1:500-1:1000 Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to human PDK1 around the phosphorylation site of serine 241(A-N-SP-F-V).

Specificity :

Recognizes human PDK1. Species crossreactivity: mouse, rat

Purity :

Purified by Immunoaffinity chromatography

Form :

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol. Purity: Purified by Immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to human PDK1 around the phosphorylation site of serine 241(A-N-SP-F-V). Specificity: Recognizes human PDK1. Species crossreactivity: mouse, rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF IHC WB

Storage Temperature :

-20°C