PDPK1, phosphorylated Ser396 (3-phosphoinositide Dependent Protein Kinase-1, hPDK1, PDK1) (APC) | US Biological P3123-02F-APC product information

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product summary

company name :

US Biological

product type :

antibody

product name :

PDPK1, phosphorylated Ser396 (3-phosphoinositide Dependent Protein Kinase-1, hPDK1, PDK1) (APC)

catalog :

P3123-02F-APC

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

APC

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

P3123-02F-APC

Product wo Prefix :

PDPK1, phosphorylated Ser396 (3-phosphoinositide Dependent Protein Kinase-1, hPDK1, PDK1) (APC)

Host :

rabbit

Product Type :

Pab

Conjugate :

APC

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research. Applications: Suitable for use in FLISA and Dot Blot. Other applications not tested. Recommended Dilution: FLISA: 1:1,000 Dot Blot: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Calc Crossreactivity :

Immunogen :

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser396 of human PDPK1 (KLH).

Specificity :

Recognizes human PDPK1 when phosphorylated at Ser393.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser396 of human PDPK1 (KLH). Specificity: Recognizes human PDPK1 when phosphorylated at Ser393. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

DB FL

Storage Temperature :

4°C Do Not Freeze

Reference :

1.Nilsen, T., et al., J. Biol. Chem. 279(6):4794-4801 (2004). 2.Collins, B.J., et al., EMBO J. 22(16):4202-4211 (2003). 3.Egawa, K., et al., J. Biol. Chem. 277(41):38863-38869 (2002). 4.Sato, S., et al., J. Biol. Chem. 277(42):39360-39367 (2002). 5.Scott, M.T., et al., EMBO J. 21(24):6771-6780 (2002).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/16/P3123-02F-APC_1.jpg