PDI (Protein Disulfide Isomerase) | US Biological P3122-11 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PDI (Protein Disulfide Isomerase)

catalog :

P3122-11

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P3122-11

Product wo Prefix :

PDI (Protein Disulfide Isomerase)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds. This process is called oxidative protein folding. Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds. Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange. This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins. Applications: Suitable for use in Western Blot, Immunofluorescence/ Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:100. Antigen retrieval in citrate buffer required. Immunofluorescence (IC): 1:50; Requires methanol permeabilization. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Pro329 of human PDI.

Specificity :

Recognizes endogenous levels of total human PDI protein at ~57kD. Species Crossreactivity: monkey, mouse and rat.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Pro329 of human PDI. Specificity: Recognizes endogenous levels of total human PDI protein at ~57kD. Species Crossreactivity: monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Huppa, J.B. & Ploegh, H.L., Cell 92: 145-148 (1998). 2. Ellgaard, L. & Ruddock, L.W., EMBO Rep. 6: 28-32 (2005). 3. Tu, B.P. & Weissman, J.S., J. Cell Biol. 164: 341-346 (2004).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/P3122-11_1.jpg

Picture4 Text :

Confocal immunofluorescent analysis of C2C12 cells using P3122-11 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).