PCTAIRE 1 | US Biological P3120-08 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PCTAIRE 1

catalog :

P3120-08

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P3120-08

Product wo Prefix :

PCTAIRE 1

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Three distinct PCTAIRE isoforms (PCTAIRE 1, PCTAIRE 2 and PCTAIRE 3) have been identified in humans and belong to the Cdk family of serine/threonine protein kinases. These proteins have a core kinase domain flanked by unique amino-and carboxy- terminal domains. Cdk proteins are known to regulate the cell cycle. All three PCTAIRE isoforms are abundantly expressed and catalytically active in post-mitotic brain, suggesting that they may function in processes other than cell division (1). PCTAIRE 1 is a cytoplasmic phosphoprotein whose kinase activity peaks in G2 and S phase (2). While one study indicates that noncovalent interactions with a regulatory component (such as a cyclin) are necessary for catalytic activity of PCTAIRE 1, others show that the monomeric protein is fully active (3). The Cdk5/p25 complex phosphorylates PCTAIRE 1 at Ser95, enhancing its kinase activity (4). Application: Suitable for use in Western Blot, Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide derived from the sequence of human PCTAIRE 1. Species sequence homology: human

Specificity :

Detects endogenous levels of total PCTAIRE 1. Species Crossreactivity: mouse and rat.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide derived from the sequence of human PCTAIRE 1. Species sequence homology: human Specificity: Detects endogenous levels of total PCTAIRE 1. Species Crossreactivity: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IP WB

Storage Temperature :

-20°C

Reference :

1. Meyerson, M., et al., EMBO J. 11: 2909- 2917 (1992). 2.Charrasse, S., et al., Cell Growth Differ. 10: 611-620 (1999). 3 Graeser, R., et al., J. Cell Sci. 115: 3479-3490 (2002). 4. Cheng, K., et al., J. Biol. Chem. 277: 31988-31993 (2002).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/23/P3120-08_1.jpg