antibody

PAX5 (Paired Box Protein Pax-5, B Cell-specific Transcription Factor, BSAP, PAX-5)

P3113-83V1

100 ug

polyclonal

domestic goat

nonconjugated

human, mouse

PAX5 (Paired Box Protein Pax-5, B Cell-specific Transcription Factor, BSAP, PAX-5)

goat

Pab

Antibodies

100 ug

IgG

Pax-5 is a 46kD, B cell specificactivator protein (BSAP). In the early stages of B cell developmemt, Pax-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20. Pax-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells. Interestingly, Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. This indicates that Pax-5 is important in B cell development, but may also have a role in proper neuronal development. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:64,000 Western Blot: 0.3-1ug/ml, human lymph and human spleen lysates observed on ~40kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide corresponding to DLEKNYPTPRTSR-C, from human PAX5, at N-terminal (NP_057953.1). Species sequence homology: mouse

Recognizes human PAX5.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to DLEKNYPTPRTSR-C, from human PAX5, at N-terminal (NP_057953.1). Specificity: Recognizes human PAX5. Species sequence homology: Mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Adams B, Dorfler P, Aguzzi A, Kozmik Z, Urbanek P, Maurer-Fogy I, Busslinger M. Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis. Genes Dev 1992 Sep;6(9):1589-607.