PARP, Cleavage Site (Asp214) (Poly ADP-Ribose Polymerase) | US Biological P3113-07D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PARP, Cleavage Site (Asp214) (Poly ADP-Ribose Polymerase)

catalog :

P3113-07D

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A46(19F4)

reactivity :

human

product information

Catalog Number :

P3113-07D

Product wo Prefix :

PARP, Cleavage Site (Asp214) (Poly ADP-Ribose Polymerase)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

3A46(19F4)

Isotype :

IgG1

Desc1 :

PARP, a 116kD nuclear poly (ADPribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICElike caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP the cleavage occurs between Asp214 and Gly215, which separates PARP’s N-terminal DNA binding domain (24kD) from its C-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:2000, incubate membrane with diluted antibody in 5% nonfat dry milk, TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic PARP peptide corresponding to C-terminal residues adjacent to Asp214 in human PARP.

Specificity :

Recognizes endogenous levels of the large fragment (89kD) of human PARP following cleavage at Asp214. Does not react with full length PARP.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3A46(19F4) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic PARP peptide corresponding to C-terminal residues adjacent to Asp214 in human PARP. Specificity: Recognizes endogenous levels of the large fragment (89kD) of human PARP following cleavage at Asp214. Does not react with full length PARP. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Satoh, M.S. and Lindahl, T. (1992) Nature 356: 356–358. 2. Lazebnik, Y.A., et al., (1994) Nature 371: 346–347. 3. Cohen, G.M. (1997) Biochem. J. 326: 1–16. 4. Nicholson, D.W., et al., (1995) Nature 376: 37–43. 5. Tewari, M., et al., (1995) Cell 81: 801–809. 6. Oliver, F.J., et al., (1998) J. Biol. Chem. 273: 33,533–33,539.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/38/P3113-07D_1.jpg