PARP (Poly ADP-Ribose Polymerase) | US Biological P3113-07 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PARP (Poly ADP-Ribose Polymerase)

catalog :

P3113-07

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P3113-07

Product wo Prefix :

PARP (Poly ADP-Ribose Polymerase)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP the cleavage occurs between Asp214 and Gly215, which separates PARP’s N-terminal DNA binding domain (24kD) from its C-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the caspase cleavage site in PARP (KLH).

Specificity :

Detects endogenous levels of full length PARP (116kD), as well as the large fragment (89kD) and small fragment (24kD) of PARP resulting from caspase cleavage. Does not crossreact with related proteins. Species Crossreactivity: Human, mouse, monkey and rat.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the caspase cleavage site in PARP (KLH). Specificity: Detects endogenous levels of full length PARP (116kD), as well as the large fragment (89kD) and small fragment (24kD) of PARP resulting from caspase cleavage. Does not crossreact with related proteins. Species Crossreactivity: Human, mouse, rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Satoh, M.S. & Lindahl, T., Nature 356: 356-358 (1992). 2. Lazebnik, Y.A., et al., Nature 371: 346-347 (1994). 3. Cohen, G.M., Biochem. J. 326: 1-16 (1997). 4. Nicholson, D.W., et al., Nature 376: 37-43 (1995). 5. Tewari, M., et al., Cell 81: 801-809 (1995). 6. Oliver, F.J., et al., J. Biol. Chem. 273: 33,533-33,539 (1998). 7. Li, J., et al., J. Biol. Chem. 277: 388-394 (2002). 8. Soldatenkov, V.A., et al., J. Biol. Chem. 277: 665-670 (2002).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/26/P3113-07_1.jpg