PARP (Poly ADP-Ribose Polymerase) | US Biological P3113-01N product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PARP (Poly ADP-Ribose Polymerase)

catalog :

P3113-01N

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

7H48(46D11)

reactivity :

human

product information

Catalog Number :

P3113-01N

Product wo Prefix :

PARP (Poly ADP-Ribose Polymerase)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H48(46D11)

Isotype :

IgG

Desc1 :

PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates PARP's amino-terminal DNA binding domain (24kD) from its carboxy-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence/Immunocytochemistry: 1:800 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Gly623 of human PARP.

Specificity :

Recognizes endogenous levels of total full-length human PARP at ~116kD and the large fragment (89kD) produced by caspase cleavage. Does not react with PARP2 or PARP3. Species Crossreactivity: monkey, mouse and rat

Purity :

Purified

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H48(46D11) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Gly623 of human PARP. Specificity: Recognizes endogenous levels of total full-length human PARP at ~116kD and the large fragment (89kD) produced by caspase cleavage. Does not react with PARP2 or PARP3. Species Crossreactivity: monkey, mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF IP WB

Storage Temperature :

-20°C

Reference :

(1) Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356–358. (2) Lazebnik, Y.A. et al. (1994) Nature 371, 346–347. (3) Cohen, G.M. (1997) Biochem. J. 326, 1–16. (4) Nicholson, D.W. et al. (1995) Nature 376, 37–43. (5) Tewari, M. et al. (1995) Cell 81, 801–809. (6) Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533–33539

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/30/P3113-01N_1.jpg