PAR2 (Proteinase Activated Receptor 2) (PE) | US Biological P3105-75F product information

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product summary

company name :

US Biological

product type :

antibody

product name :

PAR2 (Proteinase Activated Receptor 2) (PE)

catalog :

P3105-75F

quantity :

100Tests

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

7H40

reactivity :

human

product information

Catalog Number :

P3105-75F

Product wo Prefix :

PAR2 (Proteinase Activated Receptor 2) (PE)

Host :

mouse

Product Type :

Mab

Conjugate :

Category :

Antibodies

Size1 :

100Tests

Clone # USB :

7H40

Isotype :

IgG2a

Desc1 :

]Human protease-activated receptor 2 (PAR2) is one of 4 members in a unique subclass of the Class A G protein- coupled receptor superfamily. Receptor activation involves the proteolytic uncovering of a cryptic N-terminus receptor sequence that forms a tethered ligand and binds to the receptors within the extracellular loop 2. This activates the receptors to initiate multiple signaling cascades. In addition, short peptides (5-6 amino acids) synthesized based on the proteolytically revealed motif can activate the receptors without the cleavage by enzymes. PAR2 is activated by trypsin and trypsin-like enzymes, and the cleavage site has been identified at residue Arg 36. PAR2 plays an important role in the regulation of cardiovascular, gastrointestinal, and skin function. PAR2 is expressed in human vascular tissues, pancreas, kidney, colon, liver, small intestine, and some tumor cell lines (1, 2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells using HT-29 human colon adenocarcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Calc Crossreactivity :

Immunogen :

NS0 mouse myeloma cell line transfected with human PAR2, Swiss/UniProt Accession: P55085

Specificity :

Recognizes human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.

Purity :

Purified by Protein A affinity chromatgraphy from cell culture supernatant.

Form :

Supplied as a liquid in saline, 0.09% sodium azide, 0.5% BSA. Labeled with R-Phycoerythrin (PE).

Concentration :

~0.05mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 7H40 Host: mouse Source: human Concentration: ~0.05mg/ml Form: Supplied as a liquid in saline, 0.09% sodium azide, 0.5% BSA. Labeled with R-Phycoerythrin (PE). Purity: Purified by Protein A affinity chromatgraphy from cell culture supernatant. Immunogen: NS0 mouse myeloma cell line transfected with human PAR2, Swiss/UniProt Accession: P55085 Specificity: Recognizes human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C Do not freeze

Reference :

1. Macfarlane, et al., Pharmacol. Rev. 53: 245 (2001). 2. Bohm, et al., Biochem. J. 314: 1009 (1996).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/45/P3105-75F_1.jpg