PAR2 (Proteinase Activated Receptor 2) | US Biological P3105-75E product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PAR2 (Proteinase Activated Receptor 2)

catalog :

P3105-75E

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H40

reactivity :

human

product information

Catalog Number :

P3105-75E

Product wo Prefix :

PAR2 (Proteinase Activated Receptor 2)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

7H40

Isotype :

IgG2a

Desc1 :

Proteinase-Activated Receptor 2 (PAR-2) is a proteinase activated 7-transmembrane G-protein-coupled receptor. Human PAR-2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361aa receptor. PAR-2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR-2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR-2 shows 78% amino acid identity with mouse PAR-2 over the extracellular portions. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells tested with HT-29 human colon adenocarcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to human PAR-2 expressed in NS0 cells.

Specificity :

Recognizes human PAR-2. Stains human PAR2 transfectants but not irrelevant transfectants.

Purity :

Purified by Protein A affinity chromatography from tissue culture supernatant.

Form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 7H40 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein A affinity chromatography from tissue culture supernatant. Immunogen: Recombinant protein corresponding to human PAR-2 expressed in NS0 cells. Specificity: Recognizes human PAR-2. Stains human PAR2 transfectants but not irrelevant transfectants. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/36/P3105-75E_1.jpg