antibody

PAK2 (p21 Activated Kinase 2)

P2488-12H

20 ul

monoclonal

domestic rabbit

nonconjugated

8A25(C17A10)

PAK2 (p21 Activated Kinase 2)

rabbit

Mab

Antibodies

20 ul

8A25(C17A10)

IgG

The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including serines 199 and 204 of PAK1 and serines 192 and 197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation of Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation of Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). More recently identified family members including PAK4, PAK5 and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation of Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo

Synthetic peptide corresponding to the N-terminal region of human PAK2. Uniprot Accession: Q13177

Recognizes endogenous levels of total human PAK2 protein. Does not cross-react with PAK1, PAK3 or PAK4-6. Species Crossreactivity: monkey and mouse

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 8A25(C17A10) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the N-terminal region of human PAK2. Uniprot Accession: Q13177 Specificity: Recognizes endogenous levels of total human PAK2 protein. Does not cross-react with PAK1, PAK3 or PAK4-6. Species Crossreactivity: monkey and mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Knaus, U.G. and Bokoch, G.M. (1998) Int. J. Biochem. Cell Biol. 30, 857–862. 2. Daniels, R.H. et al. (1998) EMBO J. 17, 754–764. 3. King, C.C. et al. (2000) J. Biol. Chem. 275, 41201–41209. 4. Manser, E. et al. (1997) Mol. Cell. Biol. 17, 1129–1143. 5. Gatti, A. et al. (1999) J. Biol. Chem. 274, 8022–8028. 6. Lei, M. et al. (2000) Cell 102, 387–397. 7. Chong, C. et al. (2001) J. Biol. Chem. 276, 17347–17353. 8. Zhao, Z. et al. (2000) Mol. Cell. Biol. 20, 3906–3917. 9. Abo, A. et al. (1998) EMBO J. 17, 6527–6540. 10. Qu, J. et al. (2001) Mol. Cell. Biol. 21, 3523–3533.

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