PA28, alpha (Proteasome Activator 28-alpha subunit) | US Biological P1700-20 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PA28, alpha (Proteasome Activator 28-alpha subunit)

catalog :

P1700-20

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P1700-20

Product wo Prefix :

PA28, alpha (Proteasome Activator 28-alpha subunit)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28 and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionary conserved subunits of PA28; PA28alpha (PSME1), PA28beta (PSME2) and PA28gamma (PSME3) (1,2). PA28alpha and PA28beta form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28alpha/beta complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptide from foreign proteins (2). PA28gamma exists in the form of a homoheptamer and is mainly located in the nucleus. PA28gamma complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hm Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Lys13 of human PA28 alpha

Specificity :

Detects endogenous levels of total PA28 alpha protein.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Lys13 of human PA28 alpha Specificity: Detects endogenous levels of total PA28 alpha protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Dahlmann, B. (2005) Essays Biochem. 41, 31-48. 2. Rechsteiner, M. and Hill, C.P. (2005) Trends Cell Biol. 15, 27-33. 3. Zhou, P. (2006) Cell 124, 256-257. 4. Li, X. et al. (2006) Cell 124, 381-392.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/29/P1700-20_1.jpg