p70 S6 Kinase, phosphorylated (Thr389) (p70 S6K) | US Biological P1001-37L product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

p70 S6 Kinase, phosphorylated (Thr389) (p70 S6K)

catalog :

P1001-37L

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

3i22(1A5)

reactivity :

human

product information

Catalog Number :

P1001-37L

Product wo Prefix :

p70 S6 Kinase, phosphorylated (Thr389) (p70 S6K)

Host :

mouse

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

3i22(1A5)

Isotype :

IgG2a

Desc1 :

P70 S6 kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5’ oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal (1). Both isoforms lie on a mitogen-activated signaling pathway downstream of phosphoinositide 3 kinase (PI3K) and the target of rapamycin FRAP/mTOR, and distinct from the Ras/MAP kinase cascade (1). The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (1). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (1). Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo (2). Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 (4,5). Phosphorylation of this site is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI3 kinase inhibitor) and rapamycin (FRAP/mTOR inhibitor) (1,6,7). Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region (1). Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression (1,2). Another LY294002 and rapamycin sensitive phosphorylation site, Ser 371, has been shown to be an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Dr Hu Mk Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues around Thr389 of human p70 S6 kinase.

Specificity :

Recognizes endogenous levels of human p70 S6 kinase only when phosphorylated at Thr389. This antibody also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412) and possibly S6KII phosphorylated at Thr401. Species Crossreactivity: monkey, mouse, rat, D. melanogaster

Purity :

Ascites

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 3i22(1A5) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic phosphopeptide corresponding to residues around Thr389 of human p70 S6 kinase. Specificity: Recognizes endogenous levels of human p70 S6 kinase only when phosphorylated at Thr389. This antibody also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412) and possibly S6KII phosphorylated at Thr401. Species Crossreactivity: monkey, mouse, rat, D. melanogaster Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Pullen, N. and Thomas, G., (1997) FEBS Lett. 410: 78-82. 2. Weng, Q.P., et al., (1998) J. Biol. Chem. 273: 16,621-16,629. 3. Pullen, N., et al., (1998) Science 279: 707-710. 4. Alessi, D., et al., (1998) Curr. Biol. 8: 69-81. 5. Polakiewicz, R.D., et al., (1998) J. Biol. Chem. 273: 23,534-23,541. 6. Jefferies, H.B., et al., (1997) EMBO J. 16: 3,693- 3,704.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/10/P1001-37L_1.jpg