antibody

p70 S6 Kinase, phosphorylated (Thr389) (p70 S6K) discontinued

P1001-37D

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

p70 S6 Kinase, phosphorylated (Thr389) (p70 S6K) discontinued

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

p70 S6 kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal (1). Both isoforms lie on a mitogen-activated signaling pathway downstream of phosphoinositide 3 kinase (PI3K) and the target of rapamycin FRAP/ mTOR, and distinct from the Ras/MAP kinase cascade (1). The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (1). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (1). Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo (2). Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 (3,4). Phosphorylation of this site is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI3 kinase inhibitor) and rapamycin (FRAP/mTOR inhibitor) (1,5,6). Ser411, Thr421 and Ser424 lie within a Ser-Prorich region located in the pseudosubstrate region (1). Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression (1,2). Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000, Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues around Thr389 of human p70 S6 kinase.

Recognizes endogenous levels of human p70 S6 kinase only when phosphorylated at Thr389. Also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412) and possibly S6KII phosphorylated at Thr401. It will also detect some phosphorylated isoforms of PKC if present at high levels in cell extracts. Species Crossreactivity: Mouse, monkey and rat.

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues around Thr389 of human p70 S6 kinase. Specificity: Recognizes endogenous levels of human p70 S6 kinase only when phosphorylated at Thr389. Also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412) and possibly S6KII phosphorylated at Thr401. It will also detect some phosphorylated isoforms of PKC if present at high levels in cell extracts. Species Crossreactivity: Mouse, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Pullen, N. and Thomas, G. (1997) FEBS Lett. 410: 78–82. 2. Weng, Q.P., et al., (1998) J. Biol. Chem. 273: 16,621–16,629. 3. Pullen, N., et al., (1998) Science 279: 707–710. 4. Alessi, D., et al., (1998) Curr. Biol. 8: 69–81. 5. Polakiewicz, R.D., et al., (1998) J. Biol. Chem. 273: 23,534–23,541. 6. Jefferies, H.B., et al., (1997) EMBO J. 16: 3,693–3,704. 7. Aoki, M., et al., (2001) Proc. Natl. Acad. Sci. USA 98: 136–141. 8. Feng, L.X., et al., (2000) J. Biol. Chem. 275: 25,572–25,576. 9. Giallourakis, C., et al., (2000) J. Biol. Chem. 275: 29,275–29,282. 10. Kenerson, H.L., et al., (2002) Cancer Res. 62: 5,645–5,650. 11. Shioi, T., et al., (2000) EMBO J. 19: 2,537–2,548. 12. Valentinis, B., et al., (2000) J. Biol. Chem. 275: 25,451–25,459.

https://usbio-images.r.worldssl.net/prodimages/38/P1001-37D_1.jpg