p63, phosphorylated (Ser160/162) (KET, p51, p40, AIS, p73L) | US Biological P1001-34V product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

p63, phosphorylated (Ser160/162) (KET, p51, p40, AIS, p73L)

catalog :

P1001-34V

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

P1001-34V

Product wo Prefix :

p63, phosphorylated (Ser160/162) (KET, p51, p40, AIS, p73L)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription intiation site that yields a 40kD DNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the a, b and g isoforms (3,4). TAp63-a (fulllength) contains multiple serine residues followed by proline (Ser-Pro motif) that are potential cdk substrates expected to be phosphorylated in mitosis. Among these are Ser160, Ser162, Ser395, and Ser455. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser160/160 of human TAp63-a. Species sequence homology: mouse, rat, chicken and xenopus

Specificity :

Detects endogenous levels of p63 only when phosphorylated at serine 160/162.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser160/160 of human TAp63-a. Species sequence homology: mouse, rat, chicken and xenopus Specificity: Detects endogenous levels of p63 only when phosphorylated at serine 160/162. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Levine, A. J. et al. (1997) Cell 88, 323–331. (2) Waltermann, A. et al. (2003) Oncogene 22, 5686–5693. (3) Hibi, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5462–5467. (4) Yang, A. et al. (1999) Nature 398, 714–718.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/26/P1001-34V_1.jpg