p53 (Cellular Tumor Antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor Suppressor p53, TP53) | US Biological P1001-30V product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

p53 (Cellular Tumor Antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor Suppressor p53, TP53)

catalog :

P1001-30V

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13A263

reactivity :

human

product information

Catalog Number :

P1001-30V

Product wo Prefix :

p53 (Cellular Tumor Antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor Suppressor p53, TP53)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

13A263

Isotype :

IgG1

Desc1 :

p53, a 53kD protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4h) compared to that of wild-type (20min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-4ug/ml Immunoprecipitation: 4-8ug/ml Immunohistochemistry: 4-8ug/ml Immunocytochemistry: 4-8ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to aa surrounding Ser20 of human p53.

Specificity :

Recognizes human p53. Species Crossreactivity: mouse and rat.

Purity :

Purified by affinity chromatography.

Form :

Supplied as a liquid in PBS, 1mg/ml BSA, 1.5mM sodium azide, 50% glycerol.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13A263 Host: mouse Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, 1mg/ml BSA, 1.5mM sodium azide, 50% glycerol. Purity: Purified by affinity chromatography. Immunogen: Synthetic peptide corresponding to aa surrounding Ser20 of human p53. Specificity: Recognizes human p53. Species Crossreactivity: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IC IHC IP WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/44/P1001-30V_1.jpg