Ovalbumin discontinued | US Biological O8075-32 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Ovalbumin discontinued

catalog :

O8075-32

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8C6

product information

Catalog Number :

O8075-32

Product wo Prefix :

Ovalbumin discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

8C6

Isotype :

IgG1,k

Desc1 :

Ovalbumin is the main protein found in egg white, making up 60-65% of the total protein. Native chicken ovalbumin is a globular monomer with a molecular mass of 45kD. It is structurally related to both serpins and lipocalins. Applications: Suitable for use in ELISA. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/O-Ag14 myeloma cell line. Culture Medium: RPMI 1640 with 10% fetal calf serum Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Purified chicken ovalbumin adsorbed onto aluminum hydroxide gel.

Specificity :

Recognizes native chicken ovalbumin. Does not recognize denatured ovalbumin. Epitope recognition differs from that of O8075-30 and O8075-31 evidenced by lack of mutual inhibition of antigen binding.

Purity :

Purified by Protein A/G affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 8C6 Host: mouse Source: chicken Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide. Purity: Purified by Protein A/G affinity chromatography from culture supernatant. Immunogen: Ovalbumin isolated from chicken egg white, untreated. Specificity: Specific for native chicken ovalbumin (2). Cross-reactivity with egg albumin from other species has not been tested. Epitope specificity differs from those of clones 6F157 and 6F158 as determined by inhibition ELISA. Shows high affinity for OA in its native form. It does not recognize denatured OA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Nisbet AD, Saundry RH, Moir AJ, Fothergill LA, Fothergill JE (1981) The complete amino acid sequence of hen ovalbumin. Eur J Biochem 115:335-345. 2. Koch C, Jensen SS, Oster A, Houen G (1996) A comparison of the immunogenicity of the native and denatured forms of a protein. APMIS 104:115-125. 3. Houen G, Koch C (1997) A non-denaturing enzyme linked immunosorbent assay with protein preadsorbed onto aluminum hydroxide. Journal of Immunological Methods 200: 99-105 4. Kurosaka K, Chen Q, Yarovinsky F, Oppenheim JJ, Yang D (2005) Mouse cathelin-related antimicrobial peptide chemoattracts leukocytes using formyl peptide receptor-like 1/mouse formyl peptide receptor-like 2 as the receptor and acts as an immune adjuvant. J Immunol 174:6257-65.