ORC2 (Origin Recognition Complex Subunit 2) | US Biological O7050-05B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

ORC2 (Origin Recognition Complex Subunit 2)

catalog :

O7050-05B

quantity :

100 ul

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

7H46(3G6)

reactivity :

human

product information

Catalog Number :

O7050-05B

Product wo Prefix :

ORC2 (Origin Recognition Complex Subunit 2)

Host :

rat

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H46(3G6)

Isotype :

IgG2a

Desc1 :

The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near sites of initiation of DNA replication. All six ORC subunits are essential for initiation of DNA replication(1-3), and ORC may be involved in regulation of gene expression in response to stress(4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division(5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation(6-7). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk

Immunogen :

Recombinant protein corresponding to full length human ORC2.

Specificity :

Recognizes endogenous levels of total human ORC2 protein at ~78kD. Does not crossreact with other ORC subunits. Species Crossreactivity: monkey.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 7H46(3G6) Host: rat Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Recombinant protein corresponding to full length human ORC2. Specificity: Recognizes endogenous levels of total human ORC2 protein at ~78kD. Does not crossreact with other ORC subunits. Species Crossreactivity: monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF IP WB

Storage Temperature :

-20°C

Reference :

1. Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624–27630. 2. Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428–12435. 3. Gibson, D.G. et al. (2006) Genes Cells 11, 557–573. 4. Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763–776. 5. Rowles, A. and Blow, J.J. (1997) Curr Opin Genet Dev 7, 152-157. 6. DePamphilis, M.L. (2003) Gene 310, 1-15. 7. McNairn, A.J. et al. (2005) Exp Cell Res 308, 345-356.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/9/O7050-05B_1.jpg