antibody

ORC1 (Origin Recognition Complex Protein subunit 1)

O7050-02B

100 ul

monoclonal

rat

nonconjugated

7A7

The same clone is also sold as:

• Cell Signaling Technology: 4731
• Invitrogen: MA5-17025
• Abnova: MAB2489
• Santa Cruz Biotechnology: sc-23887 X, sc-23887

ORC1 (Origin Recognition Complex Protein subunit 1)

rat

Mab

Antibodies

100 ul

7A7

IgG1

The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hm Hu Mo Rt

His6-tagged recombinant human ORC1 protein.

Recognizes endogenous levels of total human ORC1 protein. Does not crossreact with other ORC subunits. Species Crossreactivity: Hamster, mouse and rat.

Ascites

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 7A7 Host: rat Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: His6-tagged recombinant human ORC1 protein. Specificity: Recognizes endogenous levels of total human ORC1 protein. Does not crossreact with other ORC subunits. Species Crossreactivity: Hamster, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1 Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624-27630. 2 Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428-12435. 3 Gibson, D.G. et al. (2006) Genes Cells 11, 557-573. 4 Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763-776. 5 Rowles, A. and Blow, J.J. (1997) Curr. Opin. Genet. Dev. 7, 152-157. 6 DePamphilis, M.L. (2003) Gene 310, 1-15. 7 McNairn, A.J. et al. (2005) Exp. Cell. Res. 308, 345-356.

https://usbio-images.r.worldssl.net/prodimages/35/O7050-02B_1.jpg