OGT (UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase 110kD Subunit, O-linked N-acetylglucosamine Transferase 110kD Subunit, O-GlcNAc Transferase Subunit p110) | US Biological O5750-01C product information

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product summary

company name :

US Biological

product type :

antibody

product name :

OGT (UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase 110kD Subunit, O-linked N-acetylglucosamine Transferase 110kD Subunit, O-GlcNAc Transferase Subunit p110)

catalog :

O5750-01C

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

product information

Catalog Number :

O5750-01C

Product wo Prefix :

OGT (UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase 110kD Subunit, O-linked N-acetylglucosamine Transferase 110kD Subunit, O-GlcNAc Transferase Subunit p110)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:64,000 Western Blot: 0.05-0.2ug/ml, observed in pancreas lysates from rat and mouse on ~110kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Ca Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to C-YEHPKDLKLSDGR, from OGT, at the internal region of the protein (NP_858058.1, NP_858059.1).

Specificity :

Recognizes OGT. Species Crossreactivity: Mouse and rat. Species sequence homology: Canine and human.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-YEHPKDLKLSDGR, from OGT, at the internal region of the protein (NP_858058.1, NP_858059.1). Specificity: Recognizes OGT. Species Crossreactivity: Mouse and rat. Species sequence homology: Canine and human. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Majumdar G, Wright J, Markowitz P, Martinez-Hernandez A, Raghow R, Solomon SS. Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1. Diabetes. 2004 Dec;53(12):3184-92.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/3/O5750-01C_1.jpg